CD11c (clone EBS-CD-011), anti-human

Source:
A BALB/c mouse was immunized with human macrophages.
Fusion partner: P3-X63-Ag8.653.



Specifications:
IntegrinαX (CD11c, leukocyte surface antigen p150/95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with β2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin αX/β2 shares similar properties of the Integrin αM/β2 in mediating adherence of neutrophils and monocytes to stimulated endothelial cells and in phagocytosis of complement coated particles. Abnormal expression of Integrin αX is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Integrin αx is present on dendritic cells, macrophages and NK-cells. Upon activation, DCs present in skin (Langerhans cells_, lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B-cells to initiate and shape the immune response.

Species reactivity:
Positive: human.

Applications:
CD11c is expressed in hairy cell leukemias, acute non-lymphocytic leukemias and some B-cell chronic lymphocytic leukemias. Marker for macrophages and NK-cells. EBS-CD-11 blocks the binding of CD11c to fibrinogen.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: Human dendritic cells. Human lymph node and tonsil.

References:
- Cabañas C, et al., Hybridoma7(2):167-76(1988).
- Cabañas C, et al., Immunol Lett.20(3):193-76 (1988).
- Zhou JQ, et al. Blood 82:800-6 (1993).
- Nicolaou, F., et al. Blood101: 4033-4041 (2003).
- Edwards, A.D. et al. J. Immunol.171: 47-60 (2003).