CD13 (clone EBS-CD-012), anti-human

Source:
A BALB/c mouse was immunized with human AML cells.
Fusion partner: P3-X63-Ag8.653.



Specifications:
EBS-CD-12 recognizes an extracellular epitope on an integral membrane glycoprotein of 150 kDa, identified as CD13 (also known as aminopeptidase-N). CD13 is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (hCoV) and human cytomegalovirus (hCMV).

Species reactivity:
Positive: human.

Applications:
CD13 forms a marker for acute myeloid leukemia.
Flow cytometry: +
Frozen section: +s
Immunofluorescence: +
Paraffin sections: +
Western blot: +

Positive control: Human lymph node, tonsil or brain.

References:
- Favaloro EJ, et al., Exp Hematol. 13:1695-701 (1993).
- Favaloro EJ, et al., Clin Chim Acta.220(1):81-90 (1993).
- Lachance C., et al. J Virol. 72(8):6511-9.(1998).
- Koch AE, et. al. Pathobiology, 58: 241-8 (1990).
- Koch AE, et. al. Am J Pathol. 138(1): 165-73.( 1991).
- Leucocyte Typing V, Schlossman SF, et. al. (eds.), Oxford University Press, Oxford, p771 (1995).
- Principe, S. et al, Proteome Res.6;11(4): 2386-96 (2012).