CD15 (Lewis X) (clone Bra4F1), anti-human

Source:
A BALB/c mouse was immunized with k562 (erythroid-myeloid leukemia cell line).
Fusion partner: SP2/0.



Specifications:
Bra4F1 reacts with CD15 (220 kDa). CD15 is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is further expressed in Reed-Sternberg cells in classic Hodgkin’s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Bra4F1 was clustered at the IVth International Workshop on Leucocyte Differentiation Antigens.

Species reactivity:
Positive: human.

Applications:
Bra4F1 can be used to identify Reed-Sternberg cells in Hodgkin’s lymphoma biopsies.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Tris/EDTA

Positive control: U937 cells, Reed-Sternberg’s cells in Hodgkin’s lymphoma.

References:
- Chorvath B. et al., Neoplasma36(6), 633-642 (1989).
- Leukocyte typing IV, Oxford Univ. Press, 868, 870 and 877 (1989).