CD15 (Lewis X) (clone FR4A5), anti-human

Source:
A BALB/c mouse was immunized with RAJI (Burkitt’s lymphoma) cells. Splenocytes were fused with mouse myeloma X63Ag8/653 cells.



Specifications:
FR4A5 reacts with CD15 (220 kDa). CD15 contains the pentasaccharide lacto-n-fucopentaoseIII and plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. It is also expressed on a widevariety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Cross reactivity has been observed with Glcα1-6glcα1-4Glc, Tn, blood group H1, and maltose.

Species reactivity:
Positive: human.

Applications:
FR4A5 can be used for enumeration of granulocytes in peripheral blood, functional studies of granulocytes (e.g. respiratory bursts, chemotaxis, phagocytosis, C5a binding), myeloid leukemia phenotyping and for identifying Reed-Sternberg cells in frozen sections of Hodgkin’s disease.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: U937 cells, Reed-Sternberg’s cells in Hodgkin’s lymphoma.

References:
- Manimala J.C. et al. Glycobiology. 17(8):17C-23C (2007).
- Gildersleeve J. et al. Glycobiology. 18(0): 746 (2008).