CD16 (clone 3G8) PE, anti-human CE-IVD

This antibody reacts with the CD16-antigen. CD16 exists as two distinct isoforms, FcγRIIIA and FcγRIIIB. In humans, FcγRIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. FcγRIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in non-human primates. Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.

Recomended usage
CD16 clone 3G8, mAb is used for identify/enumerate human antigen (FcRIII). It´s an antipan FcγRIII (CD16) whereas clone GRM1 recognizes NA2-FcRIIIb & FcRIIIa. It´s effective for direct IF staining of human tissue for FCM tests using ≤1 μg/10^6 cells.