CD16 (clone CB-16), anti-human

Source:
A BALB/c mouse was immunized with human PBL.
Fusion partner: NS-1.



Specifications:
CB16 specifically recognizes CD 16. This molecule also named low affinity Fc-receptor for IgG (FcgammaRIII) exhibits two truncated Ig-like domains and is 50-80 kDa. It is highly expressed on NK-cells, granulocytes and macrophages. CB16 binds to 15% peripheral lymphocytes of healthy donors (NK-cells), granulocytes, macrophages. CD16 represents the functional receptor structure for antibody-dependent cellular cytotoxicity.

Species reactivity:
Positive: human.

Applications:
CB16 can be used for staining of frozen tissues and in flow cytometry on NK-cells, monocytes, granulocytes.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: K-562 or U-937 cells. Lymph nodes and Tonsils.

References:
- Deaglio S. et al., Blood99(7): 2490-8 (2002).
- Zilber MT et al. Proc Natl Acad Sci U S A97(6): 2840-5 (2000).
- Wirthmueller U et al. J Exp Med.175(5): 1381-90 (1992).