CD174 (H/Lewis Y, Lewis b, Histone H1) (clone EBS-CD-049), anti-human, bovine

Source:
A BALB/c mouse was immunized with neuraminidase-treated live T-47D breast carcinoma cells.
Fusion partner: SP2/0.



Specifications:
EBS-CD-049 recognizes LeY (CD174) and to some extent Leb.EBS-CD-049 displays no cross-reactivity with blood group antigens A, B, Lea, LeX, Sialyl-Lea, Sialyl-LeX, nor with H type 1, type 2, or type 3, nor with 74 other synthetic oligo- and mono-saccharides tested (minimal reactivity exists to Globo-H). In addition, a protein conformational epitope on human and bovine histone H1 is detected (mimicry). Histones H2A, H2B, H3 and H4 are not detected.

Species reactivity:
Positive: cow, human.

Applications:
EBS-CD-049 can be used on frozen and paraffin-fixed tissues sections. It can also be used in Western blotting or histone H1.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: +
Western blotting: +

Positive control: Breast or colorectal cancer.

References:
- Christensen P.A. et al, Scand J Immunol.65(4): 362-367 (2007).
- Karsten U. et al, Differentiation, 54: 55-66 (1993)