CD176 (T-antigen TF alpha+beta) (clone EBS-CD-054), anti-human

Source:
A BALB/c mouse was immunized with neuraminidase-treated human red blood cells.
Fusion partner: X63-AG8.653.



Specifications:
EBS-CD-054 recognizes the carbohydrate epitope Thomsen-Friedenreich (asialo-glycophorin; Galβ1-3GalNAc). It is specific for both anomeric forms of this disaccharide (TFα and TFβ, including related structures on glycolipids). Immunoblotting with human acute myelogenous leukemia cells revealed a series of TF-active glycoproteins with a main band at about 155 kDa. It shows no cross-reactivity with sialylated glycophorin. Human cells normally do not carry Thomsen-Friedenreich antigen. Only after malignant transformation the antigen may appear and is thus considered a pan-carcinoma marker. EBS-CD-054 was typed at the 7th CD-Workshop.

Species reactivity:
Positive: human.

Applications:
EBS-CD-054 can be applied for the detection of cells with TF-antigens and id especially applicable for sensitive determination of neuraminidase. EBS-CD-054 can be used on frozen and paraffin-fixed tissues sections and is capable to agglutinate desialylated red blood cells.
ELISA: +
Frozen sections: +
Paraffin sections: +
Western blot: +

Positive control: KG1 cells or human colorectal carcinoma tissues.

References:
- Karsten U. et al, Hybridoma14(1): 37-44 (1995).
- Cao Y. et al, Histochem Cell Biol106: 197-207 (1996).
- Baldus SE et al, Cancer (1998), 82(6):1019-27.
- Ryder SD et al, Gastroenterology114(1): 44-9 (1998).
- Kanitakis J et al, J Clin Pathol51(8): 588-92 (1998)