CD176 (TF-antigen cluster,asialo glycophorin) (clone EBS-O-165), anti-human

Source:
A BALB/c mouse was immunized with neuraminidase-treated human red blood cells.
Fusion partner: NS-0.



Specifications:
EB-O-165 recognizes the carbohydrate epitope Thomsen-Friedenreich and especially clustered TF (asialo-glycophorin; GalNac-βGALcluster). It does not react which either bovine glycophorin or K562 cells, even after neuraminidase treatment. It has also no cross-reactivity with sialylated glycophorin.

Species reactivity:
Positive: human. Negative: cow.

Applications:
EB-O-165 can be applied for the detection of cells with clustered TF- antigens and is especially applicable for sensitive determination of neuraminidases. It reacts with the N-terminal T1/2 fragment. EB-O-165 can be used on frozen and paraffin-fixed tissue sections and is capable to agglutinate desialylated red blood cells.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: +

Positive control: Desialylated red cells.

References:
- Karsten U et al. Hybridoma14(1): 37-44 (1995).
- Cao Y. et al, Histochem Cell Biol106:197-207 (1996).
- Baldus SE et al. Cancer82(6):1019-27(1998).
- Ryder SD et al, Gastroenterology114(1): 44-9 (1998).
- Kanitakis J et al. J Clin Pathol51(8): 588-92 (1998).