CD18 (LFA-1) (clone 68-5A5), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
It recognizes a transmembrane glycoprotein of 95 kDa, identified as CD18 or intergrin-2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers. LFA-1, MAC-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54, ICCAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and IC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This Mab blocks these cellular interactions. 68-5A5 was clustered at the IIIrd International Workshop on human leucocyte differentiation antigens.Species reactivityPositive:human.Applications68-5A5 can be used for staining of frozen tissues, in flow cytometry and for blocking cell-c ell interactions.Flow cytometryFrozen sectionsFunctional studiesImmunofluorescenceParaffin sectionsWestern blot++++-+FormatProduced in tissue culture, contains no host Ig. Antibodies are affinity purified and presented in PBS with 0,02% sodium azide.Stored at 4°C-8°C, shelf life is at least 24 months after purchase.Dilution adviceFlow cytometry (0,5-1,0 μg/million cells in 0,1 ml).Functional studies (0,02-2,0 μg/ml without azide).Immunoblotting (1,0-2,0 μg/ml).Immunofluorescence (0,5-1,0 μg/ml).Immunohistology (1-2 μg/ml for 30 min at RT; an appropriate antigen retrieval method for staining formalin-fixed tissues has not been established to date).

Species reactivity:
Positive: human.

Applications:
68-5A5 can be used for staining of frozen tissues, in flow cytometry and for blocking cell-c ell interactions.
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +
Paraffin sections: -
Western blot: +

Positive control: Human PBL and tonsil.

References:
- McMichael A.J.et al., Leucocyte typing III, Oxford University Press, Oxford, (1987).