CD19 (clone CB19), anti-human

Source:
A BALB/c mouse was immunized with human PBLs.
Fusion partner: SP2/0.



Specifications:
CB19 is specific for the antigen CD19. This antigen has a MW of 120 kDa and contains a 280 residue extracellular domain and a 240 residue cytoplasmic domain. It is a critical signal transduction molecule that regulates B-lymphocyte development, activation, and differentiation. It plays a dominant role in establishing signalling thresholds for antigen receptors and other surface receptors on B-lymphocytes. This antigen is lost upon terminal differentiation to plasma cells.Species reactivityPositive:human.ApplicationsCB19 can be used for immunophenotyping of leukemia and malignant cells in frozen tissue, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunoadsorption methods.Flow cytometryFrozen sectionsImmunofluorescence+++FormatProduced in tissue culture, contains no host Ig. Antibodies are affinity purified and presented in PBS with 0,02% sodium azide.Stored at 4°C-8°C, shelf life is at least 24 months after purchase.Dilution adviceFlow cytometry (0,5-1,0 μg/million cells in 0,1 ml).Immunofluorescence (0,5-1,0 μg/ml).Immunohistology (1-2 μg/ml for 30-60 min at RT; information on a suitable antigen retrieval method for staining of formalin-fixed tissues is unavailable to date).

Species reactivity:
Positive: human.

Applications:
CB19 can be used for immunophenotyping of leukemia and malignant cells in frozen tissue, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunoadsorption methods.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Raji cells, human tonsil or lymph node.

References:
- Deaglio, S., et. al. J Immunol.160(1): 395-402 (1998)