CD19p for CAR detection CE-IVD
€0.00
In stock
SKU
IS-19-0212-
Catalog Number: PCD19-0212-25T (25 Tests) - PCD19-0212-100T (100 Tests)
Datasheet
White Paper
Request Information
Datasheet
White Paper
Request Information
Immunostep’s CD19 CAR T-Cell is an antigen-based reagent consisting of the human CD19 extracellular domain (20-291) conjugated to fluorocrhome using a proprietary technology (BrightStep®) that enhances the detection of CAR T-Cells.
Limitations of current reagents:
There are some reagents for identifying and quantifying CAR-T cells, based on fluorescent labeled (PE or FITC) recombinant CD19.
However, current reagents have limitations in separating the desired populations with sensitivity, making it more difficult for the analysts to provide an accurate and reliable result.
Those reagents that overcome this problem have the disadvantage of involving more complex and time-consuming protocols, using an amplification reagent in addition to the protein.
It is therefore necessary to introduce new simple, straightforward and easy to use reagents that are able to clearly discriminate the CAR T-Cells from the rest of populations for a more reliable analysis.
Detection of CAR T-Cells:
To meet these needs, we have developed a single reagent for direct and specific recognition of CAR T-Cells with high resolution.
This reagent allows the specialists to confirm the successful modification of T-Cells in a quality control prior infusion into the patient and to monitor them after this step.
Technology
Recombinant Fc-Tag CD19 protein (CD19p) has been labeled, by a proprietary process, with R-Phycoerythrin fluorochrome (R-PE or PE), obtaining a conjugate that is able to be recognized by CAR T-Cells and separates them efficiently without any secondary reagent (Immunostep, ref. CD19-0212).
Other manufacturers have similar products based on this fluorochrome-protein complex (e.g. ACRO Biosystems, ref. CD9-HP2H3 | ref. CD9-HF2H2 | ref. CD9-HF251) but have limitations in the separation due to bioconjugation techniques and/or the fluorochrome used.
Application: Flow Cytometry
Storage Temperature: Store in the dark at 2-8 °C
Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed
Limitations of current reagents:
There are some reagents for identifying and quantifying CAR-T cells, based on fluorescent labeled (PE or FITC) recombinant CD19.
However, current reagents have limitations in separating the desired populations with sensitivity, making it more difficult for the analysts to provide an accurate and reliable result.
Those reagents that overcome this problem have the disadvantage of involving more complex and time-consuming protocols, using an amplification reagent in addition to the protein.
It is therefore necessary to introduce new simple, straightforward and easy to use reagents that are able to clearly discriminate the CAR T-Cells from the rest of populations for a more reliable analysis.
Detection of CAR T-Cells:
To meet these needs, we have developed a single reagent for direct and specific recognition of CAR T-Cells with high resolution.
This reagent allows the specialists to confirm the successful modification of T-Cells in a quality control prior infusion into the patient and to monitor them after this step.
Technology
Recombinant Fc-Tag CD19 protein (CD19p) has been labeled, by a proprietary process, with R-Phycoerythrin fluorochrome (R-PE or PE), obtaining a conjugate that is able to be recognized by CAR T-Cells and separates them efficiently without any secondary reagent (Immunostep, ref. CD19-0212).
Other manufacturers have similar products based on this fluorochrome-protein complex (e.g. ACRO Biosystems, ref. CD9-HP2H3 | ref. CD9-HF2H2 | ref. CD9-HF251) but have limitations in the separation due to bioconjugation techniques and/or the fluorochrome used.
Application: Flow Cytometry
Storage Temperature: Store in the dark at 2-8 °C
Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed
| Is Featured? | Yes |
|---|
Write Your Own Review