CD20.4 (clone 93-1B3), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
93-1B3 binds with CD20 which is a 30/33 kDa non-glycosylated transmembrane phosphoprotein with three extensive hydrophobic regions. CD20 is involved in regulation of B-cell activation. It is expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. Plasma cells are negative. CD20 is retained on many B-cell malignancies. CD20 positive cells are also sometimes found in cases of Hodgkin’s disease, myeloma, and thymoma. 93-1B3 has been clustered at the IIIrd and Vth HLDA Workshops. Species reactivityPositive:human.Applications93-1B3 reacts with pre B-cells, resting and activated B-cells but not with plasma cells. It can be applied for characterization of leukemia and malignant cells.Flow cytometryFrozen sectionsFunctional studies+++FormatProduced in tissue culture, contains no host Ig. Antibodies are affinity purified and presented in PBS with 0,02% sodium azide.Stored at 4°C-8°C, shelf life is at least 24 months after purchase.Dilution adviceFlow cytometry (0,5-1,0 μg/million cells in 0,1 ml).Functional studies (0,02-2,0 μg/ml without azide).Immunohistology (1-2 μg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date).

Species reactivity:
Positive: human.

Applications:
93-1B3 reacts with pre B-cells, resting and activated B-cells but not with plasma cells. It can be applied for characterization of leukemia and malignant cells.
Flow cytometry: +
Frozen sections: +
Functional studies: +

Positive control: Daudi, Raji, U266, human lymphocytes. Lymph nodes and tonsils.

References:
- Cobbold, S. et al., in leucocyte typing III (ed. McMichael A.J. et al.), Oxford University Press (1987).
- Schlossman S, et al. (eds). Leukocyte Typing V, Oxford University Press, Oxford, p511-515, (1995)