CD205 (Proximal Nephrogenic Renal Antigen) (clone PN-15), anti-human, monkey, rat, horse

Source:
A BALB/c mouse was immunized with renal cortical tissue extract.
Fusion partner: Sp2/0.



Specifications:
PN-15 reacts with a lectin receptor like glycoprotein of 200 kDa (gp200), present in proximal renal tubules and on urothelium. The antigen is carbohydrate in nature. Other normal tissues that display the antigen include breast, parathyroid glands, thymus and epididymis. Among renal carcinomas 93% of primary and 84% of metastatic carcinomas are positive. Bladder cancers are also largely positive. Other tumor types include breast cancer, teratocarcinomas and parathyroid adenomas. The antigen, also called DEC-205, was assigned to CD205 at CD workshop VII. In the immune system it can facilitate tolerance to self-antigens through uptake of apoptosis derived material by dendritic cells, which in turn present fragments through MHC II and MHC I, either inducing or repressing immune responses, depending on the nature of concomitant signals.

Species reactivity:
Positive: human, monkey, rat, horse.

Applications:
PN-15 can be used in immunohistochemistry, flow cytometry, immunofluorescence and immunoblotting.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Renal carcinoma, bladder carcinoma.

References:
- Yoshida, S.O. et al, Cancer Res49: 1802-1809 (1989).
- Li, G, et al, Anticancer Res.20(4): 2773-8 (2000).
- Batchelder C.A. et al, Anat Rec (Hoboken)297(8): 1392-1406 (2014).
- Cykowski M.D. et al, Ultrastruct Pathol39(1): 69-77 (2015).
- Knez V.M. et al,J Med Case Rep8: 275 (2014).
- Christopher M.G. et al,Cytojournal.8: 7 (2011).