CD235ab (clone HIR2), anti-human
€323.00
In stock
SKU
250984
Catalog Nr.: 250984
Size: 0.1 mg
Isotype: mouse IgG2b
Applications: E, FC, IHC
Size: 0.1 mg
Isotype: mouse IgG2b
Applications: E, FC, IHC
Protein Family: Cellular Antigens, Receptors and Channels
Pathway and Disease: Immune System, Infectious Diseases, Signaling Molecules and Interaction
Description:
The CD235ab (HIR2) antibody recognizes a N-terminal homologous portion of glycophorin-A (GPA) and glycophorin-B (GPB), which are single-pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface acting as a barrier to cell fusion, therefore minimizing aggregation between red blood cells in the circulation. The CD235ab (HIR2) antibody agglutinates untreated RBC but failed to agglutinate papain-treated cells. This antibody primarly binds to GPA and weakly to GPB. CD235ab is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235ab positive whereas CD45 and CD71 negative.
Alternate Names: CD235ab, CD235a, Glycophorin-A, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GYPA, GPA, CD235B, Glycophorin-B, PAS-3, Sialoglycoprotein delta, SS-active sialoglycoprotein, GYPB, GPB
Application Notes: Purified antibody is suitable for immunohistochemistry with acetone-fixed frozen and formalin-fixed paraffin sections. E: 1:500-1:1,000; FC: 1:200-1:1,000; IHC: 1:200-1:500
Accession No.: P02724; P06028
Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
Product Citations:
Shen DC., et al. 1993. J. of Monoclonal Antibody. 9(2): 59
David M., et al. 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582.
Pathway and Disease: Immune System, Infectious Diseases, Signaling Molecules and Interaction
Description:
The CD235ab (HIR2) antibody recognizes a N-terminal homologous portion of glycophorin-A (GPA) and glycophorin-B (GPB), which are single-pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface acting as a barrier to cell fusion, therefore minimizing aggregation between red blood cells in the circulation. The CD235ab (HIR2) antibody agglutinates untreated RBC but failed to agglutinate papain-treated cells. This antibody primarly binds to GPA and weakly to GPB. CD235ab is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235ab positive whereas CD45 and CD71 negative.
Alternate Names: CD235ab, CD235a, Glycophorin-A, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GYPA, GPA, CD235B, Glycophorin-B, PAS-3, Sialoglycoprotein delta, SS-active sialoglycoprotein, GYPB, GPB
Application Notes: Purified antibody is suitable for immunohistochemistry with acetone-fixed frozen and formalin-fixed paraffin sections. E: 1:500-1:1,000; FC: 1:200-1:1,000; IHC: 1:200-1:500
Accession No.: P02724; P06028
Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
Product Citations:
Shen DC., et al. 1993. J. of Monoclonal Antibody. 9(2): 59
David M., et al. 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582.
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