CD25 (clone 143-13), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
143-13 Reacts with CD25 (55 kDa) which binds to IL-2 with low affinity, unless it associates as alpha chain with CD122 and the common gamma chain (CD132) to form the high-affinity IL-2 receptor complex. The CD25 molecule reveals three epitope regions: A, B, and C. 143-13 Recognizes epitope region B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25. 143-13 Was assigned to CD25 at the Fourth International Workshop (code A27).Species reactivity Positive:human.Applications143-13 Reacts with resting and more strongly with activated T and B lymphocytes and activated macrophages. It can be applied as a marker for cell activity. Since it blocks binding of IL-2 it is excellent for functional studies. Soluble IL2RA is excreted and serves as tumor marker for stages III and IV of stomach cancer.ELISAFlow cytometryFrozen sectionsFunctional studiesImmunofluorescenceParaffin sectionsWestern blot+++++-+FormatProduced in tissue culture, contains no host Ig. Antibodies are affinity purified and presented in PBS with 0,02% sodium azide.Stored at 4°C-8°C, shelf life is at least 24 months after purchase.Dilution adviceELISA (solid phase: 0,1-100 μg/ml; tracer: 0,001-100 μg/ml for 20 min at RT).Flow cytometry (1-2 μg/million cells for 30 min, at 4°C).Functional studies (0,02-2,0 μg/ml without azide).Immunoblotting (1-2 μg/ml).Immunofluorescence (1-2 μg/ml).Immunohistology (1-2 μg/ml for 30 min at RT; no antigen retrieval procedure is known to date for formalin-fixed tissues). Figure 1:Activated human PBL stained for CD25 (FACS).

Species reactivity:
Positive: human.

Applications:
143-13 Reacts with resting and more strongly with activated T and B lymphocytes and activated macrophages. It can be applied as a marker for cell activity. Since it blocks binding of IL-2 it is excellent for functional studies. Soluble IL2RA is excreted and serves as tumor marker for stages III and IV of stomach cancer.
ELISA: +
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +
Paraffin sections: -
Western blot: +

Positive control: PHA-stimulated human lymphocytes. Human lymph nodes and tonsils.

References:
- Knapp W. et al. Leucocyte typing IV, p. 408- 411 and p. 1080, Oxford University Press, Oxford (1989).