CD25 (clone EBS-CD-017), anti-human

Source:
A BALB/c mouse was immunized with human PBMCs.
Fusion partner: Sp2/0.



Specifications:
EBS-CD-017 reacts with CD25 (55 kDa) which associates as alpha chain with CD122 and the common gamma chain (CD132) to form the high-affinity IL-2 receptor complex. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin’s disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hairy cell leukemia. Increased levels of soluble CD25 are observed in leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. EBS-CD-017 recognizes B epitope, which is located at residue 3-104 of CD25 and does not block IL-2 binding to CD25. CD25 antibodies have been used successfully as a carrier for cytotoxic drugs enabling specific delivery to IL-2RA displaying target cells.Species reactivityPositive:human.ApplicationsEBS-CD-017 reacts with resting and more strongly with activated T and B lymphocytes and activated macrophages. ELISAFlow cytometryFrozen sectionsImmunofluorescenceParaffin sections+++―-FormatProduced in tissue culture, contains no host Ig. Antibodies are affinity purified and presented in PBS with 0,02% sodium azide.Stored at 4°C-8°C, shelf life is at least 24 months after purchase.Dilution adviceELISA (solid phase: 0,1-100 μg/ml; tracer: 0,001-100 μg/ml for 30 min at RT).Flow Cytometry (1-2 μg/million cells in 0,1 ml for 30 min, at 4°C).Immunofluorescence (1-2 μg/ml).Immunohistology (1-2 μg/ml for 30 min at RT; no suitable antigen retrieval procedure is known to date for formalin-fixed tissues).

Species reactivity:
Positive: human.

Applications:
EBS-CD-017 reacts with resting and more strongly with activated T and B lymphocytes and activated macrophages.
ELISA: +
Flow cytometry: +
Frozen section: +s
Immunofluorescence
Paraffin sections-

Positive control: PHA-stimulated human lymphocytes. Human lymph nodes and tonsils.

References:
- Yamamura T. et al, Eur J Surg168(1): 49-54 (2002).
- Lundin K. et al, Anal Biochem299(1): 92-7 (2001).
- Raivio E. et al, APMIS105(2): 108-14 (1997).
- Boutin B. et al, Neuropediatrics20(4): 202-6 (1989)