CD26 (clone 202-36), anti-human

Source:
A BALB/c mouse was immunized with a T-cell clone.
Fusion partner: NS-1.



Specifications:
202-36 Reacts with human CD26 (Dipeptidyl peptidase IV), a 110 kDa protein which occurs in the cell membrane of T-lymphocyte and is upregulated after activation. CD26 is also present on endothelial cells and also expressed on activated B-cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production. In Western blotting, this mAb reacts with only glycosylated CD26, but not with deglycosylated froms. It does not prevent ADA binding to CD26. The mAb was clustered at the VIth WLDA.Species reactivityPositive:human. Applications202-36 Can be used in frozen sections, flow cytometry, immunoblotting and immunofluorescence. Flow cytometryFrozen sectionsImmunofluorescenceParaffin sectionsWestern blot+++―+FormatProduced in tissue culture, contains no host Ig. Antibodies are affinity purified and presented in PBS with 0,02% sodium azide.Stored at 4°C-8°C, shelf life is at least 24 months after purchase.Dilution adviceFlow cytometry (0,5-1,0 μg/million cells in 0,1 ml).Immunoblotting (1-2 μg/ml).Immunofluorescence (0,5-1,0 μg/ml).Immunohistology (1-2 μg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date).

Species reactivity:
Positive: human.

Applications:
202-36 Can be used in frozen sections, flow cytometry, immunoblotting and immunofluorescence.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -
Western blot: +

Positive control: Human PBL or tonsil.

References:
- Kishimoto et al., Leucocyte typing VI. Gariand Publishing. (1997).