CD28 (clone 204-12), anti-Human, Mouse

Source:
A BALB/c mouse was immunized with human leucocytes.
Fusion partner: NS-1.



Specifications:
CD28, a glycoprotein of 44-88 kDa, is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. While 204-12 remained unclustered at the VIth International Workshop on human leukocyte differentiation antigens, the antibody has later been verified as CD28 specific.

Species reactivity:
Positive: Human, Mouse.

Applications:
204-12 can be applied for specific detection of CD28 on T-lymphocytes in peripheral blood and in frozen tissue sections and in extension for the characterization of immunodeficiency, leukemias and lymphomas. It can also be used for functional studies.
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +

Positive control: Jurkat cells or human lymph nodes and tonsils, epidermis: Langerhans cells.

References:
- Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York and London, (1997).