CD28 (clone CD28A-49), anti-human

Source:
A BALB/c mouse was immunized with rCD28.
Fusion partner: Sp2/0.



Specifications:
CD28 is the critical T-cell co-stimulatory receptor which provides the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader. CD28A-49 acts as agonist of CD28 mediated stimulation.

Species reactivity:
Positive: human.

Applications:
Demonstration of CD28 in immunofluorescence, frozen sections and for functional studies.
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +

Positive control: Jurkat cells or human lymph nodes and tonsils.

References:
- In Leucocyte typing Workshop VI. Sandilands, G. et al. Clinical and Experimental Immunology, 162(3), 516-27 (2010).
- Marti F, et al, J Immunol. 166(1):197-206(2001).
- Scharschmidt E, et al, Mol Cell Biol.24(9): 3860-73 (2004).
- Jeong SH, et al,J Virol.78(13): 6995-7003 (2004).