CD3 (clone RIV9), anti-human, mouse, rat

Source:
A BALB/c mouse was immunized with human peripheral lymphocytes.
Fusion partner: P3-X63-Ag8.653.



Specifications:
RIV9 reacts with the TCR/CD3 complex on T-cells. Primary signals received through this complex determine the specificity of immune reactions by selecting particular antigen/MHC-responsive clones. CD3 is of variable mass with five invariable chains (25-28, 20 and 16 kDa). RIV9 was used for research in vivo in humans, because it is of the unusual IgG3 subtype.

Species reactivity:
Positive: human, mouse, rat.

Applications:
RIV9 can be applied in IF and flow cytometry, in immunohistology (frozen sections only) and in particular for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
ELISA: +
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +

Positive control: Human tonsil.

References:
- Vaessen LM. et al, Transplant. Proc. 21(1 Pt 1): 1026-7 (1989).
- Ceuppens JL and van Vaeck F, Cell. Immunol. 118(1): 136-46 (1989).
- Jiskoot W. et al, J. Immunol. Methods138(2): 181-9 (1991).
- Jiskoot W. et al, J.Immunol. Methods138(2):273-83(1991)