CD3 FITC/CD8 PE/CD45 PE-Cy5/CD4 APC (clone 33-2A43, 143-44,D3/9,HP2/6), anti-human CE-IVD

The CD3 monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.
The CD8 monoclonal antibody is directed against the CD8-antigen (T8-antigen),which is expressed on human T lymphocytes. The monoclonal antibody reacts with 20-30% of human peripheral T lymphocytes.
The CD45 monoclonal antibody is directed against the CD45- antigen, defined T200 or Leucocyte Common Antigen. The antibody reacts with all cells of the haemopoietic lineage, not with cells of other lineages.
The CD4 monoclonal antibody is directed against the CD4-antigen (T4-antigen), which is expressed on human peripheral T lymphocytes and 80% of thymocytes. The monoclonal antibody reacts on a low level with human monocytes and macrophages.

Recomended usage
Immunostep’s CD3/CD8/CD45/CD4, is a monoclonal antibody intended for simultaneous detection and enumeration of lymphocytes. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 10^6 cells.