CD3-FITC FITC/CD4-PE/CD45-PE-Cy5 (clone 33-2A3; HP2/6; D3/9), anti-human RUO

The CD3 monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.
The CD4 monoclonal antibody is directed against the CD4-antigen (T4-antigen),which is expressed on human peripheral T lymphocytes and 80% of thymocytes. The monoclonal antibody reacts on a low level with human monocytes and macrophages. The monoclonal antibody does not react with B-cells ,granulocytes and thrombocytes.
The CD45 monoclonal antibody is directed against the CD45- antigen, defined T200 or Leucocyte Common Antigen. The antibody reacts with all cells of the haemopoietic lineage, not with cells of other lineages.

Recomended usage
Immunostep’s CD3/CD4/CD45, is a monoclonal antibody intended for simultaneous detection and enumeration of lymphocytes. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 10^6 cells.