CD326 (EPCAM) (clone EBS-CD-061), anti-human

Source:
A BALB/c mouse was immunized with MCF-7 human breast cancer cells.
Fusion partner: SP2/0.



Specifications:
Ep-CAM (also called ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kDa epithelial protein expressed on baso-lateral cell surfaces in very many epithelial tissues (but absent from mesothelial tissues). The 324AA have 3 potential glycosylation sites and is a transmembrane glycoprotein. The extracellular domain has a cysteine-rich repeat and a small domain with homology to nidogen. It is a homophilic cell-cell adhesion molecule (Ep-CAM). EBS-CD-061 reacts with most epithelial cells and carcinomas.

Species reactivity:
Positive: human. Negative: rat.

Applications:
It is the antibody of choice to differentiate between mesothelioma and carcinoma.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: HT29 cells or breast tumor.

References:
- Edwards DP et. al. Cancer Res46:1306-17 (1986).
- Litvinov et al. J. Cell. Biol.125:437-446 (1994).
- Tandon et al., Cancer Res.50: 3317-3321 (1990)