CD326 (EPCAM) (clone VU-1D9), anti-human, bovine

Source:
A BALB/c mouse was immunized with NCI-H69 SCLC cells.
Fusion partner: Sp2/0.



Specifications:
Ep-CAM (also called ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kDa epithelial protein expressed on baso-lateral cell surfaces in very many epithelial tissues (but absent from mesothelial tissues). The 324AA have 3 potential glycosylation sites and is a transmembrane glycoprotein. The extracellular domain has a cysteine-rich repeat and a small domain with homology to nidogen. It is a homophilic cell-cell adhesion molecule (Ep-CAM). VU-1D9 reacts with most epithelial cells and carcinomas.

Species reactivity:
Positive: cow, human. Negative: ferret, rat.

Applications:
It is the antibody of choice to differentiate between mesothelioma and carcinoma.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: HT29 cells or breast tumor.

References:
- Tsubura et al, J. Cutan. Pathol. 19: 73 (1992).
- Herlyn et al, HybridomaSuppl5: S3-S8 (1986).
- Szala et al, Proc. Nat. Acad. Sci. USA, 87: 3542-3546 (1990).
- Litvinov et al, J. Cell. Biol. 125: 437-446 (1994).
- Gaffey et al, Amer. J. Surg. Pathol. 16: 593-599 (1992).
- Tandon et al, Cancer Res. 50: 3317-3321 (1990).
- Simon et al, Proc. Nat. Acad. Sci. USA, 87: 2755-2759 (1990).
- Perez and Walker, J. Immunol. 142: 3662-3667 (1989).
- Latza et al, J. Clin. Pathol. 43: 213-219 (1990).
- Sheibani et al, Amer. J. Surg. Pathol. 15: 779-798 (1991).