CD37 (clone IPO-24), anti-human

Source:
A BALB/c mouse was immunized with spleen cells of a patient with hairy cell leukemia.
Fusion partner: P3-X63-Ag8.653.



Specifications:
IPO-24 reacts with CD37, a B lineage restricted antigen, strongly expressed throughout B-cell ontogeny from pre B-cell until plasmablasts. Plasma cells are negative. IPO-24 reacts with human B-cell lines Daudi, Raji, Namalva, Ramos and CABIL, whereas T-cell lines CEM, Molt-4, Jurkat, MT-4, H9 and non-lymphoid cell lines MeWo and A-431 are negative. IPO-24 stains B-cell follicles and the most strongly labelled elements are mantle zone lymphoid cells. Neutrophils, macrophages, thymocytes and dendritic reticulum cells are negative.

Species reactivity:
Positive: Human.

Applications:
IPO-24 is a valuable antibody for diagnostics of B-cell neoplasms different histological types, including chronic lymphocytic leukemia, hairy cell leukemia, pre B acute lymphoblastic leukemia and non-Hodgkin’s lymphoma.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Daudi, Raji, Namalva and CABIL cells, human tonsil or lymph node.

References:
- G. Moldenhauer G. et al., CD37 Workshop Panel report in: Schlossman et al., Leucocyte Typing V, B9, 544-545, (1995).
- Sidorenko S.P. et al., J. Tumor Marker Oncology5: 232 (1990).
- Sidorenko S.P. et al., Haemat. Transfusiol. 35 N4: 19-22 (1990).
- Sidorenko S.P. et al., Neoplasma 39(1):3-9 (1992).