CD4 (clone EDU-2), anti-human, chimpanzee, orangutan, stump-tailed macaque

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
EDU-2 reacts with human CD4, a 55 kDa single chain transmembrane glycoprotein that contains four extracellular immunoglobulin-like domains and is mainly expressed on T-helper lymphocytes, but also on cortical thymus cells, microglial and dendritic cells. It binds to HLA class II molecules during the interaction of CD4+ T cells with antigen-presenting cells or target cells. CD4 also serves as the primary cellular receptor for human immunodeficiency virus (HIV). EDU-2 was assigned to CD4 at the International Leucocyte Typing Workshops II and IV.

Species reactivity:
Positive: human, chimpanzee, orangutan, stump-tailed macaque. Negative: rhesus monkey.

Applications:
EDU-2 can be applied in frozen tissue sections, in immunofluorescence and in flow cytometry.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Human lymphocytes, lymph node or tonsil.

References:
- Knapp W. et. al. (eds) Leukocyte Typing IV, p314-330, Oxford University Press, Oxford, (1989).
- Reinberz EL et al. eds. Leykcocyte Typing II, Springer-Verlag, Berlin, (1985).