CD4 FITC/CD8 PE/CD3 PE-Cy5 (clone HP2/6, 143-44, UCTH-1), anti-human RUO

The CD4 antibody recognizes 60Kd MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD4 cluster and present on 54% of peripheral blood T lymphocytes, 50% of thymocytes and some malignant cells of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD4 antigen although cytoplasmic expression has been observed in monocytes/macrophages. The CD4 positive T lymphocyte subpopulation has been characterised functionally as comprising helper cells active in amplification of immune responses.
The CD8 antibody recognizes 30/32 kD MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen.
Finally, CD3 antibody recognizes 22/26/30 kD MW lymphocyte surface molecules associated with the T cell antigen receptor complex. Reacts with 85% of peripheral blood T lymphocytes, 70% of thymocytes, the majority of T cell chronic lymphocytic leukaemias, Sezary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.

Recomended usage
It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using5 μl/106 cells.
Helper/inducer lymphocytes are a subset of T lymphocytes (CD3 + ) that are CD4 + . CD3 + CD4 + counts are used to characterize and monitor some forms of immunodeficiency and autoimmune diseases.Determining counts of helper/inducer T lymphocytes can be useful in monitoring human immunodeficiency virus (HIV)-infected individuals. Individuals with HIV typically exhibit a steady decrease of helper/inducer T lymphocyte counts as the infection progresses. Suppressor/cytotoxic lymphocytes are a subset of T lymphocytes (CD3 +) that are CD8 +. CD3 + CD8 + counts are used to characterize and monitor some forms of immunodeficiency and autoimmune diseases. Suppressor/cytotoxic lymphocyte values lie outside the normal reference range in some autoimmune diseases, and in certain immune reactions such as acute graft-versus-host disease (GVHD) and transplant rejection. The CD8 + subset is elevated in many patients with either congenital or acquired immune deficiencies, such as severe combined immunodeficiency (SCID) or acquired immune deficiency syndrome (AIDS). The CD8 + cell population is often decreased in active systemic lupus erythematosus (SLE), but can also be increased in SLE patients undergoing steroid therapy. The Centers for Disease Control (CDC) recommends using reagent combinations containing CD3 antibodies for determining T-lymphocyte subsets in HIV-infected subjects.This kit CD4/CD8/CD3 reagent allows helper/inducer T lymphocytes to be identified and enumerated separately from contaminating CD3– and CD4 + monocytes.