CD40L human active recombinant protein
€295.00
In stock
SKU
600103
Protein Family: Cytokines, Cell Adhesion Molecules, CAM Ligands, Cellular Antigens
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and interaction
Alternate Names: CD40 ligand, CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF- related activation protein, TRAP, T-cell antigen Gp39, CD154, CD40 ligand, membrane form, CD40 ligand,soluble form, CD10LGDescription:
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. Recombinant Human CD40L produced in E. coli is a non-glycosylated polypeptide chain containing 149 amino acids with a MW of 16,308 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
H-CD40L is fully biologically active when compared to standard. The ED50 as determined using T-47D cells is <0.04 ug/ml.
Accession No.: P29965
MW: 16308 Da
Sequence:
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Purity:
Purity > 98% determined by reducing and non-reducing SDS-PAGE gel and analysis by RP-HPLC. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH7.5. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and interaction
Alternate Names: CD40 ligand, CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF- related activation protein, TRAP, T-cell antigen Gp39, CD154, CD40 ligand, membrane form, CD40 ligand,soluble form, CD10LGDescription:
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. Recombinant Human CD40L produced in E. coli is a non-glycosylated polypeptide chain containing 149 amino acids with a MW of 16,308 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
H-CD40L is fully biologically active when compared to standard. The ED50 as determined using T-47D cells is <0.04 ug/ml.
Accession No.: P29965
MW: 16308 Da
Sequence:
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Purity:
Purity > 98% determined by reducing and non-reducing SDS-PAGE gel and analysis by RP-HPLC. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH7.5. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
| Is Featured? | No |
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