CD40L mouse active recombinant protein
€295.00
In stock
SKU
600177
Protein Family: Cytokines, Cell Adhesion Molecules, CAM Ligands, Cellular Antigens
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: CD40 ligand, CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF- related activation protein, TRAP, T-cell antigen Gp39, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form,CD10Ig, Cd401, Tnfsf5Description:
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand binds to TNFRSF5 and stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. The soluble form derives from the membrane form by proteolytic processing. Recombinant Mouse sCD40 ligand produced in E. coli is a non-glycosylated polypeptide chain containing 149 amino acids with a MW of 16,409 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant mouse sCD40L is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of T-47D cells is 84ng/ml.
Accession No.: P27548
MW: 16409 Da
Sequence:
MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by RP-HPLC calibrated against a known standard and UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 25 ug protein lyophilized in 10 mM sodium phosphate pH7.5. Reconstitute with 0.25 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: CD40 ligand, CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF- related activation protein, TRAP, T-cell antigen Gp39, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form,CD10Ig, Cd401, Tnfsf5Description:
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand binds to TNFRSF5 and stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. The soluble form derives from the membrane form by proteolytic processing. Recombinant Mouse sCD40 ligand produced in E. coli is a non-glycosylated polypeptide chain containing 149 amino acids with a MW of 16,409 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant mouse sCD40L is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of T-47D cells is 84ng/ml.
Accession No.: P27548
MW: 16409 Da
Sequence:
MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by RP-HPLC calibrated against a known standard and UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 25 ug protein lyophilized in 10 mM sodium phosphate pH7.5. Reconstitute with 0.25 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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