CD43a (clone 111-3E9), anti-human

Source:
A BALB/c mouse was immunized with KG1 cells.



Specifications:
111-3E9 reacts with a 95/115 kDa protein on T-cells and thymocytes and a 115/135 kDa molecule on neutrophils and platelets. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. In addition, expression is altered in Wiskott Aldrich Syndrome. A proportion of AIDS patients have antibodies to CD43. A soluble form called galactoglycoprotein is present in serum. The epitope of 111-3E9 is clearly different from Bra7G.

Species reactivity:
Positive: human.

Applications:
111-3E9 can be used in immunohistology, immunofluorescence, flow cytometry and immunoblotting.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Paracortex in a tonsil or a reactive lymph node.

References:
- Stross, WP., et al., J. Clin. Path.42: 953-961 (1989).