CD44 (H-CAM) (clone 156-3C11), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
156-3C11 reacts with CD44, a type 1 transmembrane glycoprotein providing cell-cell and cell-matrix adhesion while linked to the cytoskeleton. It is involved in haematopoiesis, lymphocyte homing and activation, and tumor metastasis. It binds to fibrin, hyaluronate and other matrix proteins. On tumors CD44H is highly expressed, suggesting an important role in progression for this isoform. CD44 also forms the protein backbone of the human erythrocyte Lutheran antigen system. The epitope of 156-3C11 is resistant to (chemo)trypsin digestion and selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. 156-3C11 was clustered at the Vth HLDA Workshop.

Species reactivity:
Positive: human.

Applications:
CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: HeLa cells or paracortex in tonsil or lymph node.

References:
- Schlossman SF, et. al. Leucocyte Typing V, p1713-1719, Oxford Univ. Press (1995)