CD45 (clone 135-4C5), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes. Splenocytes were fused with mouse myeloma NS1 cells.



Specifications:
CD45 glycoproteins have various molecular weights on various cell types: B-cells 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Isoforms are produced by alternative splicing. Various isoforms are expressed differently on different lymphocytes. All hematopoietic cells express CD45 proteins except erythrocytes. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). 135-4C5 was clustered in Vienna at the IV international Workshop on human leucocyte differentiation antigens.

Species reactivity:
Positive: human.

Applications:
MAbs against CD45 can be used to differentiate lymphomas from carcinomas. While CD45RA is expressed mainly on B-cell lymphomas, CD45R is mainly expressed on T-cell lymphomas. In Hodgkin’s disease CD45 is usually not expressed. 135-4C5 requires an antigen retrieval step in paraffin sections.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blotting: +

Positive control: Toncil, Ramos, U-698, or GA-10 cells.

References:
- Alsinet, E. et al., Eur. J. Immunol. 20(12): 2801-2804, (1990).
- Knapp, W. et al., Leucocyte typing IV, p 531-536. Oxford Univ. Press. (1989).