CD45 (clone 136-4B5), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cell multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exon 4-6 spliced out). 136-4B5 was clustered in Vienna at the IV International CD workshop. The epitope of 136-4B5 is neuraminidase sensitive and when 136-4C5 binds to CD45 on anti Ig induced B-cells, it enhances their proliferation, while other anti CD45 antibodies can have the opposite effect.

Species reactivity:
Positive: human.

Applications:
MAbs against CD45 can be used to differentiate lymphomas from carcinomas. In Hodgkin’s disease CD45 is usually not expressed.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Toncil, Ramos, U-698, or GA-10 cells.

References:
- Alsinet, E. et al., Eur. J. Immunol. 20(12): 2801-2804, (1990).
- Knapp, W. et al., Leucocyte typing IV, p 531-536. Oxford Univ. Press. (1989).