CD45 (clone Bra55), anti-human

Source:
A BALB/c mouse was immunized with cells of the non-T, non-B acute lymphoblastic leukemia cell line REH 6.



Specifications:
CD45 glycoprotein have various molecular weight on various cell types: B-cells 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Isoforms are produced by alternative splicing of domains 4, 5 and 6. Various isoform are expressed differently on different lymphocytes. All hematopoietic cells express CD45 proteins except erythrocytes. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exon 4-6 spliced out).

Species reactivity:
Positive: human.

Applications:
MAbs against CD45 can be used to differentiate lymphomas from carcinomas. While CD45RA is expressed mainly on B-cell lymphomas, CD45R is mainly expressed on T-cell lymphomas. In Hodgkin’s disease CD45 is usually not expressed.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Toncil, Ramos, U-698, or GA-10 cells.

References:
- Chorváth et al., Neoplasma34(6), 685-692, (1987).
- Chorváth et al., Neoplasma35(5), 495-501, (1988).
- Chorváth et al. Leukocyte Typing IV, pp. 634-637, (1989)