CD45 (clone IBL-5/25), anti-mouse

Source:
A Wistar rat was immunized with mouse lymph node cells.
Fusion partner: Sp2/0-Ag14.



Specifications:
IBL-5/25 detects all isoforms of mouse CD45, a glycoprotein with various molecular weights on various cell types. In vitro it enhances the MHC Class II-induced adhesion of purified murine B cells. All haemtopoietic cells express CD45 except erythrocytes. Expression of CD45is necessary for signaling through the T cell receptor. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

Species reactivity:
Positive: mouse. Negative: human.

Applications:
Demonstration of mouse CD45.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Immunoprecipitation: +
Paraffin sections: +
Western blot: +

Positive control: Mouse spleen.

References:
- Peter Balogh et al, Dev. Immunol6: 179-185 (1998).
- Balázs M et al. J Immunol Methods218: 117-21 (1998)