CD45RA (clone 158-4D3), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
CD45 glycoprotein have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cell multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45 RB (exon 5, CD45RC (exon 6) and CD45R or CD45RO (exons 4-6 spliced out). 158-4D3 was clustered in Boston at the Vth International CD workshop as anti CD45RA, useful to differentiate “naïve” T-cells from activated (CD45R0+ve) T-cells, or to differentiate T-cell lymphomas (CD45R0+ve) from B-cell lymphomas (CD45RA+ve).

Species reactivity:
Positive: human.

Applications:
MAbs against CD45 can be used to differentiate lymphomas from carcinomas. In Hodgkin’s disease CD45 is usually not expressed.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Daudi, Raji, IM9 and human lymphocytes. Human lymph node and tonsil.

References:
- Schlossman S.F. et al, In Leucocyte Typing V, Oxford University Press, 386 398, (1995)