CD45RB (clone Bra11 (Bra11G)), anti-human

Source:
A BALB/c mouse was immunized with non-T, non-B CALLA positive ALL cell line REH.
Fusion partner: SP2/0.



Specifications:
CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). Bra11 reacts with CD45RB, which is expressed on mature B-lymphocytes and the majority of lymphomas and leukemia’s of B-cell origin. CD45RB is also expressed on cells of myeloid lineage.

Species reactivity:
Positive: human. Negative: rat, rhesus monkey.

Applications:
MAbs against CD45 can be used to differentiate lymphomas from carcinomas. In Hodgkin’s disease CD45 is usually not expressed.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: +
Western blot: +

Positive control: Ramos, U-698, or GA-10 cells. Tonsil.

References:
- Sedlák J, et al. Neoplasma: 36(6): 643-50 (1989).
- Knapp, W. et al., Leucocyte typing IV, p 634-637. Oxford Univ. Press. (1989).
- Schlosssman S.F. et al. Leukocyte typing V, Oxford University Press, (1995).
- Mitchell GB, et al., Eur J Immunol.29(5):1467-76 (1999).