CD48 (clone 156-4H9), anti-human

Source:
A BALB/c mouse was immunized with stimulated human PBL.
Fusion partner: NS-1.



Specifications:
CD48 is a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein, highly expressed on lymphocytes and monocytes and weakly on granulocytes. Platelets, fibroblasts, epithelium and endothelium are negative. CD48 has a cellular function in adhesion via its receptor CD2 and a role in gamma/delta T-cell recognition as an accessory molecule and forms one of the markers for detecting the GPI anchoring defect in patients with paroxysmal nocturnal hemoglobinuria (PNH). 156-4H9 was typed in Kobe, Japan at the VIth International Workshop on human leucocyte differentiation antigens.

Species reactivity:
Positive: human.

Applications:
CD48 can indicate the condition of paroxysmal nocturnal hemoglobinuria (PNH).
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Daudi, JY, Raji, Jurkat, and human lymphocytes. Human lymph node ad tonsil.

References:
- Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, New York and London, (1997).
- Yokoyama S et al. J Immunol146(7):2192-2200 (1991).
- Kwong YL et al. Am J Clin Pathol102(1):30-35 (1994).
- Sandrin MS et al..J Immunol, 151(9):4606-4613 (1993).
- Vaughan HA et al, Transplantation36: 446-450 (1983).
- Vaughan HA et al, Immunogenetics33: 113-117 (1991).