CD5 (clone BB8), anti-human

Source:
A BALB/c mouse was immunized with PHA activated human T cells.
Fusion partner: NS-1.



Specifications:
BB8 recognizes a 67 kDa protein, consistent with CD5. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T-cells areas. CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~ 10%) of diffuse large B-cell lymphoma. Among human cell lines, B-cell lines are negative (10/10), while human T-cell lines are positive (5/5). CD5 interacts with CD72.

Species reactivity:
Positive: human.

Applications:
Characterization of leukemia and lymphoma. CD5 is a marker for mantle cell lymphoma.
Flow cytometry: +
Frozen sections: -
Immunofluorescence: +
Paraffin sections: -

Positive control: 293T, Ramos or MOLT-4cells. Lymph node and tonsil.

References:
- Wang, MY et. al, Bone Marrow Transplant.9(5): 319-23 (1992).