CD50 (ICAM-3) (clone 101-1D2), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
101-1D2 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 12 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PO-anchored. 101-1D2 was provisionally clustered as CDw50 at the IV and confirmed as CD50 at the V international workshop on human leucocyte differentiation antigens.

Species reactivity:
Positive: human.

Applications:
11-1D2 is excellent for CD50 staining in paraffin sections and for flow cytometry.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: HL-60 or THP-1 cells. Lymph node and tonsil.

References:
- Vilella R, et. al. Tissue Antigens, 36(5): 203-10 (1990).
- Pino-Otin MR, et. al. J. of Immunology.154(6): 3015-24 (1995).
- Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press (1989).
- Schlossman SF, et. al. Leucocyte Typing V, p1542-1547, 2011, Oxford Univ. Press, (1993)