CD50 (ICAM-3) (clone 189-2G9), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
189-2G9 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 120 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PI-anchored. 186-2G9 has been clustered at the VIth GLDA International Workshop. 186-2D9 recognized the D1 domain of human CD50 and blocks binding of CD11a (α L-integrin) to CD50.

Species reactivity:
Positive: human.

Applications:
189-2G9 is excellent for CD50 staining in paraffin sections and for flow cytometry. In addition it can be used for functional studies.
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: HL-60 or THP-1 cells. Lymph node and tonsil.

References:
- LeukocyteTyping V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995)p. 1546-1547, 1578-1579.
- C.L. Holness, et al., J Biol Chem.270: 877-884(1995)\.
- Y. van Kooyk,et al., J Exp Med 183:1247-1252 (1996).
- Leukocyte TypingVI (T. Kishimoto, et al, eds.) GarlandPublishing, Inc., New York (1997) p.403-409