CD53 (clone 161-2), anti-human

Source:
A BALB/c mouse was immunized with stimulated human PBL.
Fusion partner: NS-1.



Specifications:
CD53 is a 33-55 kDa protein expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on B- and T-cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. 161-2 Partially inhibits T-cell proliferation induced by CD3 UCHT-1 antibody. 161-2 was typed in Kobe, Japan at the VIth International Workshop on human leucocyte differentiation antigens.

Species reactivity:
Positive: human. Negative: baboon, horse.

Applications:
Detection of monocytes and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, and B- and T-cells.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes, human tonsil and lymph node.

References:
- Kishimoto T. et al., eds. Leukocyte Typing VI, p509-514, Garland Publishing, Inc, New York and London, (1997)