CD54 (ICAM-1) (clone EBS-CD-031), anti-human

Source:
A BALB/c mouse was immunized with Raji Burkitt lymphoma cells.
Fusion partner: NS-1.



Specifications:
EBS-CD-031 was typed at the CD Workshop IV. Binding of EBS-CD-031 blocks cell adhesion through ICAM-1 as does ICAM-1 synthetic peptide JF9 (aa 367-394), suggesting the epitope of EBS-CD-031 lies between aa 367-394 (domain 1). ICAM-1 belongs to the immunoglobulin superfamily, C2 subset, is a transmembrane molecule of 90 kDa with 7 potential N-glycosylation sites. It is expressed on resting monocytes and endothelial cells and in response to inflammatory cytokines such as TNF-alpha, IL1 and IFN-gamma, can be highly upregulated on many other cells, e.g. on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes to integrin CD11a/CD18 (LFA 1) and to CD11b/CD18 (Mac 1). The interaction of CD54 with LFA 1 enhances antigen specific T-cell activation. CD54 also binds to CD43, fibrinogen, most human rhinoviruses and to Plasmodium falciparum infected erythrocytes. ICAM-1 may also be related to progression and metastasis of tumors.

Species reactivity:
Positive: human.

Applications:
EBS-CD-031 can be used in immunohistology, immunofluorescence, western blot and ICAM-1 functional studies.
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Raji cells, MOLT 4 cells, Human tonsils, lymph node.

References:
- Boyd et al., Proc. Nat. Acad. Sci.85(9), 3095-3099, (1988).
- Boyd et al. Blood73, 1896-1903, (1989).
- Wawryk et al.J Clin Pathol 44, 497-501 (1991).
- Fecondo et al., Proc. Nat. Acad. Sci.88(7), 2879-2882, (1991).