CD54 (ICAM-1) (clone F4-31C2), anti- human

Source:
A BALB/c mouse was immunized with human umbilical cord vein endothelial cells (HUVEC).
Fusion partner: X63Ag8/653.



Specifications:
F4-31C2 reacts with CD54 or ICAM1 (Intercellular Adhesion Molecule 1). ICAM1 belongs to the immunoglobulin superfamily, C2 subset, is a transmembrane molecule of 90 kDa with 7 potential N-glycosylation sites. It is expressed on resting monocytes and endothelial cells and in response to inflammatory cytokines such as TNF-alpha, IL1 and IFN-gamma, can be highly upregulated on many other cells, e.g. on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrin CD11a/CD18 (LFA 1) and to CD1b/CD18 (Mac 1). The interaction of CD54 with LFA 1 enhances antigen specific T-cell activation. CD54 also binds to CD43, fibrinogen, most human rhinoviruses and to Plasmodium falciparum infected erythrocytes. ICAM1 may also be related to progression and metastasis of tumors.

Species reactivity:
Positive: human.

Applications:
F4-31C2 can be used for identifying ICAM-1.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: Raji cells, MOLT-4 cells, Human tonsil, Lymph node.

References:
- Johnson, J.P., et al., Cluster Report: CD54, in : Knapp, W., et al. (eds), Leucocyte Typing IV, Oxford Univ. Press, pp 681-683.