CD55 (DAF) (clone 143-30), anti-human, pig

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
143-30 Reacts with CD55 or DAF (Decay Accelerating Factor). All leucocytes as well as human erythrocytes, fibroblasts, platelets, endothelial cells and neuroectodermal cells are positive for DAF, a glycosylphosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. CD55 also serves as receptor for CD97 and for echovirus and coxsackie B virus. DAF is deficient in both granulocytes and monocytes in patients with paroxysmal nocturnal haemoglobinuria. 143-30 Has been clustered in Vienna at the IVth International Workshop on human leucocyte differentiation antigens.

Species reactivity:
Positive: human, pig.

Applications:
Identify paroxysmal nocturnal haemoglobinuria.
Flow cytometry
Frozen sections: +
Immunofluorescence: +
Western blot: +

Positive control: Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes, human lymph nodes and tonsils.

References:
- Koretz, K. et al. British J. Cancer66: 810-814 (1992).
- Knapp, W. et al., Leucocyte typing IV, pp 541, 694-697, 1088. Oxford Univ.
- Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York and London, (1997).
- B.E. Loveland - in Leucocyte Typing VI - Part 6 – Known Non-Lineage CD Antigens - NL11 - CD55 Workshop Panel Report pp519-520, (1997).