CD55 (DAF) (clone F4-29D9), anti-human

Source:
A BALB/c mouse was immunized with human umbilical vein endothelial cells.
Fusion partner: X63Ag8/653.



Specifications:
F4-29D9 reacts with CD55 or DAF (Decay Accelerating Factor). All leucocytes as well as human erythrocytes, fibroblasts, platelets, endothelial cells and neuroectodermal cells are positive for DAF. F4-29D9 also recognizes an antigen on spermatozoid cells. It is a glycosylphosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. CD55 also serves as receptor for CD97 and for echovirus and coxsackie B virus. DAF is deficient in both granulocytes and monocytes in patients with paroxysmal nocturnal haemoglobinuria.

Species reactivity:
Positive: human.

Applications:
Identify paroxysmal nocturnal haemoglobinuria.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes, human lymph nodes and tonsils.

References:
- Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York and London, (1997).
- B.E. Loveland - in Leucocyte Typing VI - Part 6 – Known Non-Lineage CD Antigens - NL11 - CD55 Workshop Panel Report pp519-520, (1997).
- Ruix-Delgado, GJ et al, Hematology14: 33-7 (2009).