CD56 (N-CAM) (clone EBS-CD-032), anti-human

Source:
A BALB/c mouse was immunized with a crude membrane preparation of a small cell lung cancer.
Fusion partner: SP2/0.



Specifications:
EBS-CD-032 reacts with an extracellular domain (close to the cell membrane) of CD56/NCAM. Three isoforms of NCAM are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species of 180 kDa and 145 kDa. The 125 kDa isoform is not recognized by EBS-CD-032. EBS-CD-032 recognizes NCAM in retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell lung carcinomas. NCAM is also expressed on some mesodermally derived tumors (a.o. rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Species reactivity:
Positive: human.

Applications:
Differential diagnosis of neuroectodermally derived tumors like small cell lung cancer and NK/T-cell lymphomas.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin section: Citrate
Western blotting: +

Positive control: Cerebellum, pancreas, neuroblastoma.

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