CD6 (clone EBS-CD-005), anti-human

Source:
A BALB/c mouse was immunized with cells of a human CD8+ human CTL clone.
Fusion partner: SP2/0.



Specifications:
EBS-CD-005 recognizes the CD6 molecule, a single chain transmembrane glycoprotein of 120 kDa, which is expressed on the majority of mature T-cells an mature thymocytes. A B-cell subset is weakly positive and B-CLL’s may also be reactive. CD6 is a type 1 transmembrane glycoprotein that is tyrosine phosphorylated during TCR-mediated T-cell activation. CD6 shows significant homology to CD5. Antibodies to CD6 are used to deplete T-cells from bone marrow transplants to prevent graft versus host disease.

Species reactivity:
Positive: human.

Applications:
Characterization of leukemia and lymphoma.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: 293T, Ramos or MOLT-4 cells, lymph node and tonsil.

References:
- Yssel et al., Cell. Immunol.105:161(1987).
- Kamoun et al., J. Immunol.127: 987 (1981).
- Reinherz et al., Proc. Nat. Acad. Sci.79:6047(1982)