CD61 (clone EBS-CD-034), anti-human

Source:
A BALB/c mouse was immunized with stimulated human PBMC’s.
Fusion partner: NS-1.



Specifications:
EBS-CD-034 reacts with a complex of CD41 and CD61, i.e. alpha IIb integrin and the integrin beta chain. The MW of the GPIIIa is 105 kDa unreduced and 90 kDa reduced. The integrin beta 3 chain can also form a complex called the vitronectin receptor with integrin alpha V: the CD51/CD61 complex. Ligands are fibrinogen, fibronectin, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The CD51/CD61 complex is also found on endothelial cells, some B-cells, monocytes/macrophages and tumor cells.

Species reactivity:
Positive: human.

Applications:
In an autosomal recessive disorder called Glanzmann thrombasthenia, GPIIIb/IIIa is either missing or the receptor function is defective causing bleeding because platelets fail to aggregate. Found by immunohistology the best marker for megakaryoblastic/cytic leukemias and idiopathic thrombocythemia. EBS-CD-034 inhibits platelet aggregation.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: U937, KG1a, HEL cells, human platelets in lymph nodes or tonsils.

References:
- Burns et al., Cell 45, 269 280 (1986). McMichael AJ et al. (eds) Leukocyte Typing III, Oxford University Press, Oxford, (1987). Schlossman S. et al. (eds) Leukocyte Typing V, Oxford University Press, Oxford, (1995).